Characterization and sequence analysis of extended-spectrum-{beta}-lactamase-encoding genes from Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates collected during tigecycline phase 3 clinical trials
| Autor: | C Hal, Jones, Margareta, Tuckman, David, Keeney, Alexey, Ruzin, Patricia A, Bradford |
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| Rok vydání: | 2008 |
| Předmět: |
Reverse Transcriptase Polymerase Chain Reaction
Minocycline Bacterial Infections Microbial Sensitivity Tests biochemical phenomena metabolism and nutrition bacterial infections and mycoses Tigecycline beta-Lactamases Anti-Bacterial Agents Klebsiella pneumoniae Clinical Trials Phase III as Topic Mechanisms of Resistance Drug Resistance Bacterial polycyclic compounds Escherichia coli bacteria Humans Isoelectric Focusing Proteus mirabilis DNA Primers |
| Zdroj: | Antimicrobial agents and chemotherapy. 53(2) |
| ISSN: | 1098-6596 |
| Popis: | In concert with the development of novel beta-lactams and broad-spectrum cephalosporins, bacterially encoded beta-lactamases have evolved to accommodate the new agents. This study was designed to identify, at the sequence level, the genes responsible for the extended-spectrum-beta-lactamase (ESBL) phenotypes of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates collected during the global tigecycline phase 3 clinical trials. PCR assays were developed to identify and clone the bla(TEM), bla(SHV), bla(OXA), and bla(CTX) genes from clinical strains. Isolates were also screened for AmpC genes of the bla(CMY), bla(ACT), bla(FOX), and bla(DHA) families as well as the bla(KPC) genes encoding class A carbapenemases. E. coli, K. pneumoniae, and P. mirabilis isolates with ceftazidime MICs ofor =2 microg/ml were designated possible ESBL-producing pathogens and were then subjected to a confirmatory test for ESBLs by use of Etest. Of 272 unique patient isolates, 239 were confirmed by PCR and sequencing to carry the genes for at least one ESBL, with 44% of the positive isolates harboring the genes for multiple ESBLs. In agreement with current trends for ESBL distribution, bla(CTX-M)-type beta-lactamase genes were found in 83% and 71% of the ESBL-positive E. coli and K. pneumoniae isolates, respectively, whereas bla(SHV) genes were found in 41% and 28% of the ESBL-positive K. pneumoniae and E. coli isolates, respectively. Ninety-seven percent of the E. coli and K. pneumoniae isolates were tigecycline susceptible (MIC(90) = 2 microg/ml), warranting further studies to define the therapeutic utility of tigecycline against strains producing ESBLs in a clinical setting. |
| Databáze: | OpenAIRE |
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