Optical Voltage Sensing Using DNA Origami
| Autor: | Elisa A, Hemmig, Clare, Fitzgerald, Christopher, Maffeo, Lisa, Hecker, Sarah E, Ochmann, Aleksei, Aksimentiev, Philip, Tinnefeld, Ulrich F, Keyser |
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| Rok vydání: | 2018 |
| Předmět: |
Ion Transport
Letter Biosensing Techniques DNA Equipment Design single-molecule FRET Nanostructures Electricity Fluorescence Resonance Energy Transfer Nanotechnology Nucleic Acid Conformation nanocapillary DNA nanotechnology sense organs Electrodes optical voltage measurements coarse-grained simulations Fluorescent Dyes |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6992 |
| Popis: | We explore the potential of DNA nanotechnology for developing novel optical voltage sensing nanodevices that convert a local change of electric potential into optical signals. As a proof-of-concept of the sensing mechanism, we assembled voltage responsive DNA origami structures labeled with a single pair of FRET dyes. The DNA structures were reversibly immobilized on a nanocapillary tip and underwent controlled structural changes upon application of an electric field. The applied field was monitored through a change in FRET efficiency. By exchanging the position of a single dye, we could tune the voltage sensitivity of our DNA origami structure, demonstrating the flexibility and versatility of our approach. The experimental studies were complemented by coarse-grained simulations that characterized voltage-dependent elastic deformation of the DNA nanostructures and the associated change in the distance between the FRET pair. Our work opens a novel pathway for determining the mechanical properties of DNA origami structures and highlights potential applications of dynamic DNA nanostructures as voltage sensors. |
| Databáze: | OpenAIRE |
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