| Autor: |
Y, Hsu, W, Chang, P C, Newell, J D, Gross |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Biochimica et biophysica acta. 1447(1) |
| ISSN: |
0006-3002 |
| Popis: |
We previously isolated several 'promoter-trap' transformants in which insertion of a promoterless beta-galactosidase gene into the genome caused expression of beta-galactosidase in specific cell types. The upstream flanking region was rescued from one transformant specifically expressing beta-galactosidase in prespore cells. We sequenced the promoter of the gene that is fused in-frame with lacZ and characterised a negative element that inhibits expression in pstO cells (a subtype of prestalk cells). Gel-retardation assays show that a developmentally regulated factor(s) recognises and binds to this element. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
