| Autor: |
Lia, Giuseppe, Praly, Elise, Ferreira, Helder, Stockdale, Chris, Tse-Dinh, Yuk Ching, Dunlap, David, Croquette, Vincent, Bensimon, David, Owen-Hughes, Tom |
| Jazyk: |
angličtina |
| Rok vydání: |
2006 |
| Předmět: |
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| Popis: |
The Snf2 family represents a functionally diverse class of ATPase sharing the ability to modify DNA structure. Here we use a magnetic trap and an Atomic Force Microscope to monitor the activity of a member of this class: the RSC complex. This enzyme causes transient shortenings in DNA length involving translocation of typically 400 bp within 2 seconds resulting in the formation of a loop whose size depends on both the force applied to the DNA and the ATP concentration. The majority of loops decrease in size within a time similar to that with which they are formed suggesting that the motor has the ability to translocate in different directions. Loop formation is also associated with the generation of negative DNA supercoiling. These observations support the idea that the ATPase motors of the Snf2 family proteins act as DNA translocases specialised to generate transient distortions in DNA structure. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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