Co-localization of multiple antigens and specific DNA. A novel method using methyl methacrylate-embedded semithin serial sections and catalyzed reporter deposition
| Autor: | M, Mueller, K, Wacker, W F, Hickey, E B, Ringelstein, R, Kiefer |
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| Rok vydání: | 2000 |
| Předmět: |
Plastic Embedding
Chimera Histological Techniques DNA Immunohistochemistry Rats Animals Genetically Modified Sural Nerve Technical Advance Bone Marrow Rats Inbred Lew Image Processing Computer-Assisted Animals Humans Methacrylates Tissue Distribution Antigens In Situ Hybridization Bone Marrow Transplantation |
| Zdroj: | The American journal of pathology. 157(6) |
| ISSN: | 0002-9440 |
| Popis: | Co-localization of proteins and nucleic acid sequences by in situ hybridization and immunohistochemistry is frequently difficult as the process necessary to detect the target structure of one technique may negatively affect the target of the other. Morphological impairment may also limit the application of the two techniques on sensitive tissue. To overcome these problems we developed a method to perform in situ hybridization and immunohistochemistry on semithin sections of methyl methacrylate-embedded tissue. Microwave-stimulated antigen retrieval, signal amplification by catalyzed reporter deposition, and fluorescent dyes were used for both techniques, yielding high sensitivity and excellent morphological preservation compared to conventional paraffin sections. Co-localization of in situ hybridization and immunohistochemistry signals with high morphological resolution was achieved on single sections as well as on adjacent multiple serial sections, using computerized image processing. The latter allowed for the co-localization of multiple antigens and a specific DNA sequence at the same tissue level. The method was successfully applied to radiation bone marrow chimeric rats created by transplanting wild-type Lewis rat bone marrow into TK-tsa transgenic Lewis rats, in an attempt to trace and characterize TK-tsa transgenic cells. It also proved useful in the co-localization of multiple antigens in peripheral nerve biopsies. |
| Databáze: | OpenAIRE |
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