Assessment of adulteration of soybean proteins in dairy products by 2D microchip-CE device

Autor: Ruige, Wu, Zhiping, Wang, Ying Sing, Fung, Daphne Yen Peng, Seah, William Shu-Biu, Yeung
Rok vydání: 2014
Předmět:
Zdroj: Electrophoresis. 35(11)
ISSN: 1522-2683
Popis: To determine the adulteration of soybean proteins in dairy product, a microchip-CE device was developed to isolate selected fraction of soybean and milk proteins in pI range from 5.5 ∼ 7.0 by 1D IEF, followed by ITP/CZE in the embedded capillary for preconcentration, separation and UV detection at 280 nm. Compared to IEF-CZE without ITP preconcentration, the enhancement factor (EF) in detection of soybean proteins was 20 times. Adulteration of 0.1% soybean protein in total dairy proteins can be detected in less than 10 min.
Databáze: OpenAIRE