| Autor: |
Hossain, Hassanpour, Ali, Kadivar, Pejman, Mirshokraei, Hassan, Nazari, Azita, Afzali, Maryam, Badisanaye |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Veterinary Research Forum |
| ISSN: |
2008-8140 |
| Popis: |
Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli cells were isolated from testes of 10 lambs. After culture and 3 passages, they were treated with mild (39 ˚C) and severe (42 ˚C) heat stress for 6 hr. The results showed a significant reduction in the percentage of live cells under severe heat stress compared to the control group (32 ˚C), (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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