| Autor: |
Jin-miao, Qu, Jie, You, Hai-guang, Liu, Qi-di, Huang, Gui-long, Guo |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery. 16(1) |
| ISSN: |
1671-0274 |
| Popis: |
To investigate the effect of 5-fluorouracil (5-FU) on the expression of ATP-binding cassette superfamily G member 2 (ABCG2) in human colon cancer cell SW480.SW480 cells were treated with various concentrations of 5-FU. CCK8 assay was utilized to detect the 5-FU IC50 to SW480 cells. Positive expression of ABCG2 was detected by flow cytometry, and mRNA expression of ABCG2 was detected by real time polymerase chain reaction (RT-PCR).The 5-FU IC50 to SW480 cells increased as the drug concentration increased (P0.05). Flow cytometry revealed that positive expression rate of ABCG2 in normal SW480 cells (group A) was (6.26±0.86)%. Immediately after treatment with 5-FU for 48 hours, the positive expression rate of ABCG2 (group B) was (3.43±1.18)% (P0.05). In the second passage of cells after treatment with 5-FU for 48 hours, the positive expression rate of ABCG2 (group C) was (12.91±3.42)% (P0.05). The mRNA expression of ABCG2 detected by RT-PCR was in accordance with the results from flow cytometry.Expression of ABCG2 in SW480 cells can be affected by various concentrations of 5-FU. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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