Interleukin-3 and phorbol esters induce different patterns of immediate-early gene expression in an interleukin-3 dependent cell line
| Autor: | J A, McCubrey, L S, Steelman, J P, McKearn |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Proto-Oncogene Proteins c-jun
Receptors Antigen T-Cell Ornithine Decarboxylase Resting Phase Cell Cycle Cell Line Immediate-Early Proteins Histones Proto-Oncogene Proteins c-myc Mice Bacterial Proteins Proto-Oncogene Proteins Animals Cycloheximide Early Growth Response Protein 2 Early Growth Response Protein 1 Granulocyte-Macrophage Colony-Stimulating Factor Receptors Antigen T-Cell gamma-delta Blotting Northern Actins DNA-Binding Proteins Proto-Oncogene Proteins c-raf Genes ras Gene Expression Regulation Dactinomycin RNA Tetradecanoylphorbol Acetate Interleukin-3 Tumor Suppressor Protein p53 Proto-Oncogene Proteins c-fos Cell Division Half-Life Transcription Factors |
| Zdroj: | Oncogene research. 6(1) |
| ISSN: | 0890-6467 |
| Popis: | The myeloid interleukin-3 (IL-3) dependent cell line, FDC-P1, enters the G0 stage of the cell cycle after IL-3 deprivation for 24 hr. The expression of 13 protooncogenes and immediate-early genes was compared with 4 "control" genes after the addition of either IL-3 or phorbol myristate acetate (PMA) to IL-3-deprived cells. mRNA transcripts encoding c-myc and the T-cell receptor c-gamma gene were induced to high levels only after IL-3 addition, whereas c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 were induced transiently only after PMA addition. The remaining genes (fra-1, p53, jun-D, c-Ha-ras, c-Ki-ras, c-raf, beta-actin, ornithine decarboxylase, and histone 2B) were detected after culture with either IL-3 or PMA. When cells were serum- and IL-3-deprived, c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 were detected after exposure to either serum or PMA. Moreover, culture with cycloheximide and PMA resulted in superinduction of these genes, whereas cycloheximide and IL-3 addition did not. mRNAs encoding these genes had half-lives of 10-20 min after PMA treatment, whereas that of beta-actin was longer (greater than 2 hr), suggesting that short mRNA half-lives contributed to the transient nature of these genes. Although c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 expression can be detected in IL-3-dependent cells after exposure to either PMA or serum, these genes were not detected after IL-3 addition, which allows cell-cycle progression. These results document the existence of IL-3 and PMA-responsive genes and demonstrate that IL-3 and protein kinase C agonists can induce distinct patterns of gene expression. |
| Databáze: | OpenAIRE |
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