Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay
| Autor: | L J, van Doorn, A, Verschuuren-van Haperen, A, Burnens, M, Huysmans, P, Vandamme, B A, Giesendorf, M J, Blaser, W G, Quint |
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| Rok vydání: | 1999 |
| Předmět: |
Hot Temperature
Base Sequence Geography Molecular Sequence Data Australia Genetic Variation Campylobacter Bacteriology Campylobacter coli Polymerase Chain Reaction United States GTP Phosphohydrolases Campylobacter jejuni Genes Bacterial Sequence Homology Nucleic Acid Campylobacter Infections Humans Sequence Alignment Phylogeny Switzerland Netherlands |
| Zdroj: | Journal of clinical microbiology. 37(6) |
| ISSN: | 0095-1137 |
| Popis: | Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni, C. coli, C. lari, and C. upsaliensis. Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species. |
| Databáze: | OpenAIRE |
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