| Autor: |
Cristina, Ortega-Villasante, Ángel, Barón-Sola, Margarita, Toledo-Basantes, Flor, Martínez, Luis E, Hernández |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2202 |
| ISSN: |
1940-6029 |
| Popis: |
Autophagy constitutes an essential process triggered by oxidative stress that enables cells to recycle damaged biomolecules and organelles, which is eventually traced by immunodetection with anti-ATG8. In parallel with autophagy induction, carbon metabolism in Chlamydomonas reinhardtii under abiotic stress is diverged toward lipid biosynthesis and lipid droplet accumulation, which can be analyzed by a simple thin-layer chromatography and in vivo staining with the fluorescent probe BODIPY 493/503. We show the responses in Chlamydomonas cells exposed to mercury or cadmium (0-50 μM doses), as examples of oxidative stress-mediated changes in autophagy and lipid metabolism, monitored with the procedures described in this report. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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