Standardizing for microsatellite length in comparisons of genetic diversity

Autor:	Rémy J, Petit, Marie-France, Deguilloux, Joëlle, Chat, Delphine, Grivet, Pauline, Garnier-Géré, Giovanni G, Vendramin
Rok vydání:	2005
Předmět:	Magnoliopsida Tracheophyta Gene Frequency Models Genetic Mutation DNA Chloroplast Genetic Variation Regression Analysis Microsatellite Repeats
Zdroj:	Molecular ecology. 14(3)
ISSN:	0962-1083
Popis:	Mutation rates at microsatellites tend to increase with the number of repeats of the motif, leading to higher levels of polymorphism at long microsatellites. To standardize levels of diversity when microsatellites differ in size, we investigate the relationship between tract length and variation and provide a formula to adjust allelic richness to a fixed mean number of repeats in the specific case of chloroplast microsatellites. A comparison between 39 loci from eight species of conifers (where chloroplast DNA is paternally inherited) and 64 loci from 12 species of angiosperms (where chloroplast DNA is generally predominantly maternally inherited) indicates that the greater allelic richness found in conifers remains significant after controlling for number of repeats. The approach stresses the advantage of reporting variation in number of repeats instead of relative fragment sizes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9b81ec8ebc526005a0ed5a77ff958137 https://pubmed.ncbi.nlm.nih.gov/15723680 Zobrazit plný text záznamu Plný text