Structure-activity relationship study of human interleukin-3. Identification of residues required for biological activity by site-directed mutagenesis
| Autor: | N A, Lokker, N R, Movva, U, Strittmatter, B, Fagg, G, Zenke |
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| Rok vydání: | 1991 |
| Předmět: |
Base Sequence
Blotting Western Molecular Sequence Data Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay DNA Gene Expression Regulation Bacterial Binding Competitive Polymerase Chain Reaction Receptors Interleukin-3 Recombinant Proteins Structure-Activity Relationship Escherichia coli Mutagenesis Site-Directed Tumor Cells Cultured Humans Electrophoresis Polyacrylamide Gel Interleukin-3 Amino Acid Sequence Plasmids |
| Zdroj: | The Journal of biological chemistry. 266(16) |
| ISSN: | 0021-9258 |
| Popis: | Recombinant human interleukin-3 (rhuIL-3) variants were generated by site-directed mutagenesis and expression in Escherichia coli. Amino acid deletions and substitutions were made in the previously identified epitopes of two huIL-3-specific neutralizing monoclonal antibodies (mAbs). The rhuIL-3 variants were analyzed for their ability to bind to the IL-3 receptor and to induce the proliferation of the human IL-3-dependent cell line M-O7. Several deletion mutants spanning the epitopes of these neutralizing mAbs indicated the importance of residues Pro33 and Leu34 for biological activity. Further, substitution of Pro33 with Asn (Asn33) showed an enhanced proliferative activity (4-fold) and a moderate increase in receptor binding (2-fold) compared to wild-type (wt) rhuIL-3. The most remarkable change, however, was seen with variant Gly33, which showed a 14-fold increase in promoting the growth of M-O7 cells without a significant modification in its receptor binding capacity. In contrast, substitution of Leu34 with Gly (Gly34) yielded an IL-3 variant that had a 25-fold decreased receptor binding capacity and proliferative activity, while Glu34 had properties similar to wild-type rhuIL-3. Analysis of the binding of these variants to different rhuIL-3-specific monoclonal antibodies suggested that no major modification had occurred in their conformations. These results indicate that both residues, Pro33 and Leu34, play a critical role in modulating the activity of rhuIL-3. |
| Databáze: | OpenAIRE |
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