| Autor: |
T M, Williams, J, Wu, S, Bassinger, G D, Montoya, J, Yee, B B, Griffith, J, Kearns, M, McKeen, S, Birkos, I S, Gourley, M, Kamoun |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Tissue antigens. 55(5) |
| ISSN: |
0001-2815 |
| Popis: |
Several methods for low-resolution class I typing of potential bone marrow donors are available. The National Marrow Donor Program (NMDP) has initiated pilot projects for large-scale DNA-based class I typing to initially characterize donors. Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) screening of 3,500 NMDP potential donors suggested the presence of variants of known HLA-B*15 variants in 3 donors. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Sequencing revealed 3 alleles differing from known HLA-B*15 alleles by nucleotide substitutions resulting in predicted novel HLA-B antigens. The new alleles occur in distinct ethnic groups. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
