Isolation in Italy of feline immunodeficiency virus (FIV)

Autor:	C, Buonavoglia, M, Tempesta, M G, Pennisi, L, Di Trani, F, Marsilio, F, Titti, O, Catarsini, M, Compagnucci
Rok vydání:	1991
Předmět:	Cell Survival Antibodies Monoclonal Fluorescent Antibody Technique Immunodeficiency Virus Feline Recombinant Proteins Cytopathogenic Effect Viral Italy Feline Acquired Immunodeficiency Syndrome Cats Concanavalin A Leukocytes Animals Interleukin-2 Cells Cultured
Zdroj:	Microbiologica. 14(2)
ISSN:	0391-5352
Popis:	The Authors report the isolation in Italy of Feline Immunodeficiency Virus (FIV) from a cat inoculated with whole blood from a naturally FIV infected cat. The virus was isolated in feline circulating leucocytes cultured in RPMI medium and stimulated with concanavalin-A and recombinant human interleukin-2. The infected cultures showed a characteristic cytopathic effect (ballooning degeneration, giant cell formation, cell death) and a specific fuorescence using FIV-positive cat serum and monoclonal antibodies against FIV. Furthermore, the culture supernatants contained magnesium-dependent reverse transcriptase activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9b4fe2f3d6266aae824a248c25eafb74 https://pubmed.ncbi.nlm.nih.gov/1649960 Zobrazit plný text záznamu