A new organotypic model containing dermal-type macrophages
| Autor: | Nicolas, Bechetoille, Hortense, Vachon, Amandine, Gaydon, Aurelie, Boher, Thomas, Fontaine, Evelyne, Schaeffer, Marion, Decossas, Valérie, André-Frei, Christopher G, Mueller |
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| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Tissue Scaffolds Tumor Necrosis Factor-alpha Macrophages Cell Membrane Cytoplasmic Vesicles Cell Culture Techniques Antigens Differentiation Myelomonocytic Receptors Cell Surface Dermis HLA-DR Antigens Fibroblasts Microspheres Monocytes Interleukin-10 Phagocytosis Antigens CD Humans Lectins C-Type Cell Adhesion Molecules Skin |
| Zdroj: | Experimental dermatology. 20(12) |
| ISSN: | 1600-0625 |
| Popis: | Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC-SIGN/CD209 and HLA-DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti-inflammatory cytokine IL-10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage-fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages. |
| Databáze: | OpenAIRE |
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