Characterisation of two distinct HKT1-like potassium transporters from Eucalyptus camaldulensis
Autor: | D J, Fairbairn, W, Liu, D P, Schachtman, S, Gomez-Gallego, S R, Day, R D, Teasdale |
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Rok vydání: | 2000 |
Předmět: |
DNA
Complementary Xenopus Molecular Sequence Data Plant Roots Membrane Potentials Gene Expression Regulation Plant Escherichia coli Animals Protein Isoforms Tissue Distribution Amino Acid Sequence Cation Transport Proteins Plant Proteins Eucalyptus Plants Medicinal Dose-Response Relationship Drug Plant Stems Sequence Homology Amino Acid Symporters Reverse Transcriptase Polymerase Chain Reaction Genetic Complementation Test Sodium Membrane Proteins Sequence Analysis DNA Electrophysiology Plant Leaves RNA Plant Mutation Oocytes Potassium Female Carrier Proteins Sequence Alignment |
Zdroj: | Plant molecular biology. 43(4) |
ISSN: | 0167-4412 |
Popis: | Potassium is an essential macronutrient in higher plants. It plays an important physiological role in stoma movements, osmoregulation, enzyme activation and cell expansion. The demand for potassium can be substantial, especially when the plant concerned is a Eucalyptus tree in excess of 50 m tall. We have isolated two cDNAs, EcHKT1 and EcHKT2, from Eucalyptus camaldulensis (river red gum) which are expressed in leaves, stems and roots. These encode potassium transporter polypeptides with homology to the wheat K+-Na+ symporter, HKT1. EcHKT1 and EcHKT2 both complemented the K+-limited growth of an Escherichia coli K+-uptake-deficient triple mutant. EcHKT1 and EcHKT2 also mediated Na+ and K+ uptake when expressed in Xenopus oocytes. A comparison of the EcHKT1 and EcHKT2 sequences and their transport properties indicated that these cDNAs represent two K+ transporters with distinct functional characteristics. The functional and structural conservation between these two E. camaldulensis genes and the wheat HKT1 suggests that they play an important, albeit elusive, physiological role. |
Databáze: | OpenAIRE |
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