| Autor: |
R D, Stout, J, Suttles, J, Xu, I S, Grewal, R A, Flavell |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Journal of immunology (Baltimore, Md. : 1950). 156(1) |
| ISSN: |
0022-1767 |
| Popis: |
The expression of the ligand for CD40 (CD40L) is critical for induction of T cell-dependent Ab responses. To examine how critical the expression of CD40L is for induction of cell-mediated immune responses, the ability of T cells from CD40L knockout mice to activate macrophage effector function was assessed. CD4+ T cells from CD40L-knockout mice were fourfold less effective than +/+ T cells in activating the nitric oxide response in allogeneic macrophages. CD40L-knockout T cells that were fixed with paraformaldehyde after a 6-h activation period, a time point at which CD40L dominates the macrophage-activating capability of the T cell, could activate neither macrophage production of inflammatory cytokines (TNF-alpha) nor generation of reactive nitrogen intermediates. After 24 h of activation, however, both CD40L-knockout and +/+ T cells could induce similar but weak responses from the macrophages. This study demonstrates that animals deficient in CD40L expression display a deficiency in T cell-dependent macrophage-mediated immune responses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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