F0F1-ATPase activity regulated by external links on beta subunits

Autor:	Jie, Cheng, Xiao-ai, Zhang, Yao-Gen, Shu, Jia-Chang, Yue
Rok vydání:	2009
Předmět:	Protein Subunits Proton-Translocating ATPases Adenosine Triphosphate Catalytic Domain Electron Spin Resonance Spectroscopy Antibodies Monoclonal Spin Labels Biosensing Techniques Holoenzymes Protein Structure Tertiary
Zdroj:	Biochemical and biophysical research communications. 391(1)
ISSN:	1090-2104
Popis:	F(o)F(1)-ATPase activity is regulated by external links on beta subunits with different molecular weight. It is inhibited when anti-beta subunit antibody, streptavidin and H9 antibody link on the beta subunits successively, but is activated when virus was binded. Western blotting indicated that the employed anti-beta antibody target was on the non-catalytic site of the beta subunit. Furthermore, an ESR study of spin-labeled ATP (SL-ATP) showed that the affinity of ATP to the holoenzyme increases with increasing external links on the beta subunits. This simple regulation method may have great potential in the design of rapid, free labeled, sensitive and selective biosensors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9a02c6f4cb74c5b3d1bbd5e207b7fd8c https://pubmed.ncbi.nlm.nih.gov/19900413 Zobrazit plný text záznamu Full Text from ScienceDirect