| Autor: |
R C, Zangar, M, Hernandez, T A, Kocarek, R F, Novak |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
BioTechniques. 18(3) |
| ISSN: |
0736-6205 |
| Popis: |
Poly(A) tail length is important in the stability and translation of mRNA. We describe procedures for the rapid and reproducible analysis of poly(A) tail length of a single mRNA species contained in a sample of total hepatic RNA. A short 3' fragment of a specific mRNA is prepared by RNase H digestion of the targeted mRNA region annealed to a short DNA oligonucleotide. The length of the poly(A) tail of the 3' fragment is then determined by running the sample on a polyacrylamide gel, by electrophoretic transfer, by probing with a radiolabeled cDNA and by comparing the size of the detected region with a specific RNA ladder or a DNA ladder. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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