| Autor: |
Ryosuke, Munakata, Alexandre, Olry, Tomoya, Takemura, Kanade, Tatsumi, Takuji, Ichino, Cloé, Villard, Joji, Kageyama, Tetsuya, Kurata, Masaru, Nakayasu, Florence, Jacob, Takao, Koeduka, Hirobumi, Yamamoto, Eiko, Moriyoshi, Tetsuya, Matsukawa, Jérémy, Grosjean, Célia, Krieger, Akifumi, Sugiyama, Masaharu, Mizutani, Frédéric, Bourgaud, Alain, Hehn, Kazufumi, Yazaki |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Proc Natl Acad Sci U S A |
| ISSN: |
1091-6490 |
| Popis: |
Plants produce ∼300 aromatic compounds enzymatically linked to prenyl side chains via C–O bonds. These O-prenylated aromatic compounds have been found in taxonomically distant plant taxa, with some of them being beneficial or detrimental to human health. Although their O-prenyl moieties often play crucial roles in the biological activities of these compounds, no plant gene encoding an aromatic O-prenyltransferase (O-PT) has been isolated to date. This study describes the isolation of an aromatic O-PT gene, CpPT1, belonging to the UbiA superfamily, from grapefruit (Citrus × paradisi, Rutaceae). This gene was shown responsible for the biosynthesis of O-prenylated coumarin derivatives that alter drug pharmacokinetics in the human body. Another coumarin O-PT gene encoding a protein of the same family was identified in Angelica keiskei, an apiaceous medicinal plant containing pharmaceutically active O-prenylated coumarins. Phylogenetic analysis of these O-PTs suggested that aromatic O-prenylation activity evolved independently from the same ancestral gene in these distant plant taxa. These findings shed light on understanding the evolution of plant secondary (specialized) metabolites via the UbiA superfamily. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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