| Autor: |
Chen-Chen, Feng, Jian-Yu, Xiao, Li-Li, Shi, Ruo-Yang, Zhang, Min, Xue, Yan-Jun, Zhou, Cheng-Yin, Huang, Qing, Chen |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(3) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the indentification method of samples mistyped as O phenotype and to explore the precision transfusion strategy.The blood samples from donors and patients admitted in our center from 2018 to 2019 was collected. The samples with O phenotype suspected subtypes were further determined by tube test, adsorption-elution test, etc. Molecular testing was used to sequence the related blood type genes of the subjects.Among 14 subjects misjudged as O, 11 different genotypes were identified, in which 3 blood donors were Ael02/O02, Bel03/O02, and one para-Bombay with B101/O02 (FUT1: h3h3; FUT2: SeThe phenotypes of Ael, Bel, Aw and para-Bombay subtypes are easily misjudged as type O. Molecular technology is helpful to identify the genotype of subtypes, and the corresponding transfusion strategies could be reasonably performed.误判O型的疑难血型鉴定及安全输血策略的研究.综合探讨多种误判O型的疑难血型鉴定及安全输血策略.收集2018-2019年本中心接收的无偿献血者和临床患者疑难血型复检标本，对表型为O型但疑似亚型标本使用试管法、吸收放散法等进一步验证；并采用分子生物学方法对受检者的相关血型基因进行检测.14例表型被误判为O型的受检者中，共鉴定出11种基因型，其中无偿献血者3例，ABO血型基因型分别为Ael02/O02、Bel03/O02及1例类孟买B101/O02（FUT1: h3h3; FUT2: SeAel、Bel、Aw、类孟买等多种亚型的表型易被误判为O型。分子生物学手段有助于鉴定亚型基因型，合理安排相应的输血策略. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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