| Autor: |
Wei-Jiang, Liu, Xue, Li, Yuan-Lin, Liu, Peng, Wang, Yue, Fan, Tian-Yi, Teng, Bo-Qian, Bai, Yue, Tong, Wei, Zhang, Yi, Zhang |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 26(1) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the effect of MicroRNA-3963(miR-3963) on the adipogenic differentiation of mouse bone-derived mesenchymal stem cells(MSC).MSCs were isolated from C57BL/6 mice bone fragment and transfected with miR-3963 mimic, miR-3963 inhibitor and negative control. The expression of miR-3963 and transfection efficiency were detected by q-PCR. These transfected cells were induced to adipocytes and stained with oil red O after 14 days culture. q-PCR and Western blot were used to detect the expression of adipogenic differentiation marker genes C/EBPα and PPARγ at transcriptional level and protein level.The results of q-PCR revealed that miR-3963 expression level was up-regulated after transfection with miR-3963 mimic (P0.0001), and down-regulated after transfection with miR-3963 inhibitor (P0.0001). After oil red staining, overexpression of miR-3963 in MSCs could promote the formation of lipid droplet. The q-PCR and Western blot analyses showed the significant increase of expression of adipogenic marker genes C/EBPα and PPARγ in MSC transfected with miR-3963 mimic. Additionally, compared with the control group, miR-3963 inhibitor could decrease adipogenic differentiation of MSC.miR-3963 can regulate and promote adipogenic differentiation of mouse bone-derived MSC. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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