Upregulation of basolateral small conductance potassium channels (KCNQ1/KCNE3) in ulcerative colitis
| Autor: | Adel, Al-Hazza, John, Linley, Qadeer, Aziz, Malcolm, Hunter, Geoffrey, Sandle |
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| Rok vydání: | 2015 |
| Předmět: |
Colon
Cell Membrane Human colonic crypts digestive system diseases Article KCNQ1/KCNE3 channels Membrane Potentials Up-Regulation Ulcerative colitis Potassium Channels Voltage-Gated KCNQ1 Potassium Channel Potassium Humans Colitis Ulcerative Intestinal Mucosa Patch clamp Ion Channel Gating Cells Cultured |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 1090-2104 |
| Popis: | Background Basolateral K+ channels hyperpolarize colonocytes to ensure Na+ (and thus water) absorption. Small conductance basolateral (KCNQ1/KCNE3) K+ channels have never been evaluated in human colon. We therefore evaluated KCNQ1/KCNE3 channels in distal colonic crypts obtained from normal and active ulcerative colitis (UC) patients. Methods KCNQ1 and KCNE3 mRNA levels were determined by qPCR, and KCNQ1/KCNE3 channel activity in normal and UC crypts, and the effects of forskolin (activator of adenylate cyclase) and UC-related proinflammatory cytokines on normal crypts, studied by patch clamp recording. Results Whereas KCNQ1 and KCNE3 mRNA expression was similar in normal and UC crypts, single 6.8 pS channels were seen in 36% of basolateral patches in normal crypts, and to an even greater extent (74% of patches, P |
| Databáze: | OpenAIRE |
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