| Autor: |
H, Rosa, E R, Parise, F R, Paranhos, A L, Braga, A S, Freitas, A N, Hidalgo, M B, Mor |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Arquivos de gastroenterologia. 28(1) |
| ISSN: |
0004-2803 |
| Popis: |
In the present study we propose a standardization of the method for the induction of fibrosis of the liver in rats by weekly administration of CCl4 by the intragastric route. The present method is a variation of that described by Proctor and Chatamra. The initial CCl4 dose is 0.04 ml (X dose) but the subsequent doses are calibrated using a 5% variation in animal weight as a reference point. For a weight gain of 5% or more, the successive doses are progressively increased by 50 to 150% (1.5X, 2X, 2.5X). When there is no variation in weight or loss in less than 5%, the previous dose is maintained. For a weight loss of more than 5%, the CCl4 dose is decreased by 50%. Consistent fibrosis if obtained as early as during the 8th week. Collagen content increases progressively from the 8th to the 12th week, reaching on average a level eight-fold higher than that observed in the control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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