CD14
| Autor: | D, Brandt, E, Sohr, J, Pablik, A, Schnabel, F, Kapplusch, K, Mäbert, J H, Girschick, H, Morbach, F, Thielemann, S R, Hofmann, C M, Hedrich |
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| Rok vydání: | 2018 |
| Předmět: |
Inflammation
Inflammasomes Blotting Western Interleukin-1beta Lipopolysaccharide Receptors Osteomyelitis Bacterial Infections DNA Methylation Real-Time Polymerase Chain Reaction Bone and Bones Monocytes CARD Signaling Adaptor Proteins Gene Expression Regulation Case-Control Studies Chronic Disease NLR Family Pyrin Domain-Containing 3 Protein Humans |
| Zdroj: | Clinical immunology (Orlando, Fla.). 196 |
| ISSN: | 1521-7035 |
| Popis: | The pathophysiology of chronic nonbacterial osteomyelitis (CNO) remains incompletely understood. Increased NLRP3 inflammasome activation and IL-1β release in monocytes from CNO patients was suggested to contribute to bone inflammation. Here, we dissect immune cell infiltrates and demonstrate the involvement of monocytes across disease stages. Differences in cell density and immune cell composition may help to discriminate between BOM and CNO. However, differences are subtle and infiltrates vary in CNO. In contrast to other cells involved, monocytes are a stable element during all stages of CNO, which makes them a promising candidate in the search for "drivers" of inflammation. Furthermore, we link increased expression of inflammasome components NLRP3 and ASC in monocytes with site-specific DNA hypomethylation around the corresponding genes NLRP3 and PYCARD. Our observations deliver further evidence for the involvement of pro-inflammatory monocytes in the pathophysiology of CNO. Cellular and molecular alterations may serve as disease biomarkers and/or therapeutic targets. |
| Databáze: | OpenAIRE |
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