Cloning of upstream sequences responsible for cell cycle regulation of the Nicotiana sylvestris CycB1;1 gene

Autor:	C, Tréhin, I O, Ahn, C, Perennes, F, Couteau, E, Lalanne, C, Bergounioux
Rok vydání:	1998
Předmět:	Recombinant Fusion Proteins Cell Cycle Molecular Sequence Data Restriction Mapping Sequence Analysis DNA Cyclin B Plants Toxic Gene Expression Regulation Plant Genes Reporter RNA Plant Tobacco RNA Messenger Cloning Molecular Cyclin B1 Promoter Regions Genetic Cells Cultured Glucuronidase
Zdroj:	Plant molecular biology. 35(5)
ISSN:	0167-4412
Popis:	To understand the mechanisms involved in the regulation of the mitotic cyclin B Nicta; CycB1;1 expression, we have cloned the Nicotiana sylvestris cyclin gene, Nicsy; CycB1;1, whose coding sequence is homologous to that of Nicta;CycB1;1 cDNA. The structure and the function of its 5'-flanking region, 1149 bp upstream of the first start codon, was analysed. By producing stably transformed cells of a synchronized culture with the Nicsy;CycB1;1 promoter/beta-glucuronidase (gus) reporter gene fusion, we demonstrate that the 1149 bp promoter fragment mediates a gus transcriptional oscillation, indistinguishable from that of endogenous Nicsy;CycB1;1 cyclin B transcripts. Transient GUS activity in BY-2 protoplasts reveals that promoter activity is considerably reduced by shortening the 5'-flanking region to 538 or 320 bp. Furthermore, the 320 bp fragment no longer mediates the observed transcriptional regulation of the 1149 bp Nicsy;CycB1;1 promoter in BY-2 protoplasts isolated from synchronized cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::98dc15a87c9564452f3c240c9e294159 https://pubmed.ncbi.nlm.nih.gov/9349288 Zobrazit plný text záznamu Full text from SpringerLink