| Autor: |
Sylvie, Gory-Fauré, Julie, Delaroche, Camille, Cuveillier, Christian, Delphin, Isabelle, Arnal |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2430 |
| ISSN: |
1940-6029 |
| Popis: |
Neuronal microtubules have long been known to contain intraluminal particles, called MIPs (microtubule inner proteins), most likely involved in the extreme stability of microtubules in neurons. This chapter describes a cryo-electron microscopy-based assay to visualize microtubules containing neuronal MIPs. We present two protocols to prepare MIPs-containing microtubules, using either in vitro microtubule polymerization assays or extraction of microtubules from mouse hippocampal neurons in culture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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