HPLC/MS
| Autor: | Nahla, Ayoub, Nadia, Badr, Saeed S, Al-Ghamdi, Safaa, Alsanosi, Abdullah R, Alzahrani, Ashraf B, Abdel-Naim, Khaled A, Nematallah, Noha, Swilam |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Wound Healing antioxidant Plant Extracts Salvadoraceae Anti-Inflammatory Agents Salvadora persica L wound healing Antioxidants Mass Spectrometry Article oral ulcer Rats Oxidative Stress Random Allocation angiogenesis Adhesives Animals Rats Wistar HPLC-ESI-QTOF-MS-MS analysis Oral Ulcer Chromatography High Pressure Liquid Acetic Acid anti-inflammatory |
| Zdroj: | Nutrients |
| ISSN: | 2072-6643 |
| Popis: | Salvadora persica L. (S. persica, Siwak) is an ethnic plant that is widely used for improving oral hygiene. This study aimed to provide a phytochemical profiling of S. persica ethyl acetate fraction (SPEAF) and to evaluate the healing activity of a muco-adhesive formula of the fraction against acetic acid-induced oral ulcers in rats. HPLC-ESI-QTOF-MS-MS analysis of SPEAF resulted in the tentative identification of 56 metabolites containing fatty acids (23%), urea derivatives (10.5%) and sulphur compounds (10%), in addition to several amides, polyphenols and organic acids (6.5%, 5% and 2%, respectively). For the first time, 19 compounds were identified from S. persica. In vitro and in vivo experiments indicated that the extract is non-toxic. SPEAF exhibited superior healing activities compared to both the negative and positive control groups on days 7 and 14 of tongue ulcer induction. This was confirmed by histopathological examinations of haematoxylin and eosin-stained (H&E) and Masson’s trichrome-stained tongue sections. Moreover, SPEAF showed potent anti-inflammatory activities, as evidenced by the inhibited expression of interleukin-6 (IL-6) and tumour necrosis alpha (TNF-α). Moreover, SPEAF exhibited potent antioxidant activity, as it prevented malondialdehyde (MDA) accumulation, reduced glutathione (GSH) depletion and superoxide dismutase (SOD) exhaustion. SPEAF significantly enhanced hydroxyproline tongue content and upregulated collagen type I alpha 1 (Col1A1) mRNA expression. SPEAF also improved angiogenesis, as shown by the increased mRNA expression of the angiopoietin-1 (Ang-1). In conclusion, S. persica has a wide range of secondary metabolites and ameliorates acetic acid-induced tongue ulcers in rats. This can be attributed, at least partly, to its anti-inflammatory, antioxidant, procollagen and angiogenic activities. These findings provide support and validity for the use of S. persica as a traditional and conventional treatment for oral disorders. |
| Databáze: | OpenAIRE |
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