| Autor: |
Salanee, Kandandapani, Cheau Y, Tan, Adawiyah S, Shuib, Saad, Tayyab |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Protein and peptide letters. 23(6) |
| ISSN: |
1875-5305 |
| Popis: |
The influence of buffer composition on the conformational stability of native and calciumdepleted Bacillus licheniformis α-amylase (BLA) was investigated against guanidine hydrochloride (GdnHCl) denaturation using circular dichroism, fluorescence and UV-difference spectroscopy. Differential effect of buffer composition on GdnHCl denaturation of BLA was evident from the magnitude of these spectral signals, which followed the order: sodium phosphateTris-HClHEPESMOPS. These effects became more pronounced with calcium-depleted BLA. Sephacryl S-200 gel chromatographic results showed significant BLA aggregation in the presence of 6 M GdnHCl. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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