Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales

Autor: Tomas, Fessl, Joel A, Crossley, Daniel, Watkins, Marek, Scholz, Matthew A, Watson, Tara, Sabir, Sheena E, Radford, Ian, Collinson, Roman, Tuma
Rok vydání: 2021
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2168
ISSN: 1940-6029
Popis: Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10
Databáze: OpenAIRE