| Autor: |
V, Ehrhardt, H, Baadenhuijsen, S, Brenna, M, Browne, L, Garcia-Beltrán, K, Hellsing, W R, Külpmann, K, Paschen, C, Pascual-Mostaza, P, Pei |
| Jazyk: |
němčina |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Wiener klinische Wochenschrift. Supplementum. 192 |
| ISSN: |
0300-5178 |
| Popis: |
We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.6% (K+) and less than 1.7% (Cl-). The linear range of the assays were at least 80 to 200 mmol/l (Na+), 1.5 to 17 mmol/l (K+) and about 30 to at least 200 mmol/l (Cl-). The comparisons with routine flame atomic emission spectrometry and coulometry showed a satisfactory agreement of the test results. The "enzymatic" assays are insensitive to even grossly elevated levels of bilirubin and lipids (sodium, potassium, and chloride assays), NH4+ (potassium assay) and amylase (chloride assay). Interference by various drugs was not detected. Since the new methods can easily be adapted to photometric clinical chemistry instruments, they represent a valuable alternative to the use of ion-selective electrodes, flame atomic emission spectrometry and coulometry. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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