| Autor: |
S W, Singer, A P, Reddy, J M, Gladden, H, Guo, T C, Hazen, B A, Simmons, J S, VanderGheynst |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Journal of applied microbiology. 110(4) |
| ISSN: |
1365-2672 |
| Popis: |
This work aimed to characterize microbial tolerance to 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid that has emerged as a novel biomass pretreatment for lignocellulosic biomass. Enrichment experiments performed using inocula treated with [C2mim][OAc] under solid and liquid cultivation yielded fungal populations dominated by Aspergilli. Ionic liquid-tolerant Aspergillus isolates from these enrichments were capable of growing in a radial plate growth assay in the presence of 10% [C2mim][OAc]. When a [C2mim][OAc]-tolerant Aspergillus fumigatus strain was grown in the presence of switchgrass, endoglucanases and xylanases were secreted that retained residual enzymatic activity in the presence of 20% [C2mim][OAc]. The results of the study suggest that tolerance to ionic liquids is a general property of the Aspergilli. Tolerance to an industrially important ionic liquid was discovered in a fungal genera that is widely used in biotechnology, including biomass deconstruction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text