Development of Stemness in Cancer Cell Lines Resistant to the Anticancer Effects of Zoledronic Acid
| Autor: | Akira, Yoshiyama, Takeshi, Morii, Kouki, Ohtsuka, Hiroaki, Ohnishi, Takashi, Tajima, Takayuki, Aoyagi, Kazuo, Mochizuki, Kazuhiko, Satomi, Shoichi, Ichimura |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Osteosarcoma Lung Neoplasms Bone Density Conservation Agents Diphosphonates Blotting Western Imidazoles Apoptosis Bone Neoplasms Mice SCID Xenograft Model Antitumor Assays Zoledronic Acid Mice Drug Resistance Neoplasm Mice Inbred NOD Carcinoma Non-Small-Cell Lung Biomarkers Tumor Neoplastic Stem Cells Tumor Cells Cultured Animals Humans Cell Proliferation |
| Zdroj: | Anticancer research. 36(2) |
| ISSN: | 1791-7530 |
| Popis: | Drug resistance is closely related to cancer cell stemness, that is acquired along with resistance to various anticancer agents. However, this has not been investigated as a potential mechanism underlying cancer cell resistance to zoledronate, that is used to suppress bone metastasis.Zoledronate-resistant A549 lung cancer and MG63 osteosarcoma cell lines were established by repeated treatment with sub-lethal concentrations of zoledronate. Expression levels of the stem cell marker NANOG, cMYC, octamer-binding transcription factor 4, and sex-determining region Y-box 2 were evaluated and sphere formation was compared between parental and resistant cell lines. Tumourigenicity was assessed in vivo.Stem cell marker expression was up-regulated and sphere formation was enhanced in resistant compared to parental cells and showed greater tumour formation capacity in mice.Repeated treatment of malignant tumour cell lines with zoledronate, induces the development of drug resistance and stemness. |
| Databáze: | OpenAIRE |
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