| Autor: |
Wei, Shu, Yong, Guo, Shisong, Fang, Tangbing, Cui |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Wei sheng yan jiu = Journal of hygiene research. 33(6) |
| ISSN: |
1000-8020 |
| Popis: |
To study the preparation methods of polyclonal antibody of chymopapain with higher titre.Three types of antigens-native chymopapain, chymopapain inactivated by H2O2 and inactivated by iodoacetic acid were injected into different male rabbits by hypodermic injections and by intravenous injection. The blood samples were obtained from veins in ear to test antisera after the 3rd injection, the used methods were Western blotting and ELISA. The data was analyzed by the SAS system.The antisera titre of two inactivated antigens were obviously higher than that of nature antigen (P0.05), while there was no significant difference between the two inactivated antigens. Intravenous injection can obviously increase the ability of immunoresponse and raise antisera titre rapidly.The inactivated antigens have better immunogenicity than native chymopapain. As compared with iodoacetic acid, using H2O2 was an economical and effective method. The combination method of hypodermic injections and intravenous injection can raise antisera titre effectively and shorten the period of antibody preparation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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