| Autor: |
V M, Vagabov, L V, Trilisenko, I N, Shchipanova, L A, Sibel'dina, I S, Kulaev |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Mikrobiologiia. 67(2) |
| ISSN: |
0026-3656 |
| Popis: |
The dynamics of the content and the degree of polymerization of polyphosphates (PPs) during the growth of Saccharomyces cerevisiae VKM Y-1176 was studied by chemical methods and P NMR spectroscopy. The overall PP content of Saccharomyces cerevisiae cells growing in orthophosphate-sufficient medium was found to increase until glucose was exhausted in the medium. In the early logarithimic phase, the degree of polymerization of PPs (high-polymeric PPs in particular) fell drastically. Further cultivation of yeast in the glucose-depleted medium led to a decrease in the cellular content of all PP fractions without any considerable shortening of their chain length; by the 24th h, high-polymeric PPs accumulated in the cells. Shortening of PPs against a background of their intense synthesis in yeast cells growing in glucose-and orthophosphate-sufficient medium can be explained by the involvement of so far unknown mechanisms of activation of polyphosphate-depolymerizing enzymes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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