Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons

Autor: J Tabitha, Hees, Angelika B, Harbauer
Rok vydání: 2022
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2431
ISSN: 1940-6029
Popis: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.
Databáze: OpenAIRE