| Autor: |
Shuming, Zhang, Bianbian, Li, Ke, Du, Tingting, Liang, Mengyuan, Dai, Wenxin, Huang, Huizhi, Zhang, Yihui, Ling, Huidong, Zhang |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Biochimie. 168 |
| ISSN: |
1638-6183 |
| Popis: |
Among human four Y-family DNA polymerases, hPol ι is exceptionally error-prone in DNA synthesis. 6 mA plays significant roles in epigenetic regulation of numerous biological processes. Nonetheless, its effects on DNA replication by hPol ι is still unclear. In this work, we found that 6 mA and Hyp, the intermediate of 6 mA, inhibited the replication of DNA by hPol ι. 6 mA lost priority in extension beyond 6 mA:T pair, partially reducing dTTP incorporation efficiency and inhibiting next-base extension. Hyp was prone to dCTP incorporation and extension beyond Hyp:C instead of Hyp:T pair. Statistically, 6 mA primarily reduced the burst incorporation rate (k |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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