Role of PumB antitoxin as a transcriptional regulator of the PumAB type-II toxin-antitoxin system and its endoribonuclease activity on the PumA (toxin) transcript

Autor: K C, Hernández-Ramírez, M I, Valle-Maldonado, J A, Patiño-Medina, S, Calo, I E, Jácome-Galarza, V, Garre, V, Meza-Carmen, M I, Ramírez-Díaz
Rok vydání: 2022
Zdroj: Molecular genetics and genomics : MGG.
ISSN: 1617-4623
Popis: The PumAB type-II toxin-antitoxin (TA) system is encoded by pumAB genes that are organized into an operon. This system is encoded by the pUM505 plasmid, isolated from a Pseudomonas aeruginosa clinical strain. The pumA gene encodes a putative RelE toxin protein (toxic component), whereas the pumB gene encodes a putative HTH antitoxin protein. The expression of the PumAB system in Escherichia coli confers plasmid stability. In addition, PumA toxin overexpression in P. aeruginosa possesses the capability to increase bacterial virulence, an effect that is neutralized by the PumB antitoxin. The aim of this study was to establish the mechanism of regulation of the PumAB toxin-antitoxin system from pUM505. By an in silico analysis of the putative regulatory elements, we identified two putative internal promoters, P
Databáze: OpenAIRE
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