| Autor: |
N, Meissner, S, Kochs, J, Coutelle, F, Kussebi, C, Baumgarten, H, Løwenstein, G, Kunkel, H, Renz |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 29(5) |
| ISSN: |
0954-7894 |
| Popis: |
The aim of the study was to analyse early effects of specific immunotherapy (SIT) on immune functions in cat-allergic patients.Immunological responses of peripheral blood mononuclear cells from eight cat-allergic patients were analysed before and after SIT in comparison with 11 nonallergic controls. Cells were stimulated in vitro with either bacterial superantigen, mitogen, or cat allergen. Production of IL-12 and TH1/TH2 cytokines was analysed by ELISA and lymphocyte subset distribution was assessed by flow-cytometry.We found a significantly reduced secretion of IL-12 (P0.05) from cells of allergic individuals compared with the controls. This finding was associated with significantly lower IFN-gamma production after stimulation with allergen (P0.05) that did not increase during SIT. However, no significant differences were seen after stimulation with mitogen indicating an allergen specific IFN-gamma secretion response in allergic individuals. Prior to SIT IL-5 production was significantly higher in cells of allergic donors stimulated with allergen0.005 or mitogen (0.05). After reaching the maintenance dose for SIT, allergen-induced IL-5 production returned to normal levels, whereas it remained elevated after stimulation with mitogen. These changes were associated with a reduced frequency of CD45 RO T cells following SIT.These results suggest that SIT exerts early effects on allergen-specific T-cell responses with selective inhibition of the up-regulated TH2 immune response. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
