| Autor: |
C I, Westacott, R M, Atkins, P A, Dieppe, C J, Elson |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
The Journal of rheumatology. 21(9) |
| ISSN: |
0315-162X |
| Popis: |
To determine whether the previously observed enhanced susceptibility of osteoarthritic (OA) cartilage to tumor necrosis factor-alpha (TNF-alpha) induced degradation could be due to differences in receptor expression.Using specific monoclonal antibodies flow cytometry was used to identify and quantify 2 TNF-alpha receptors on the surface of chondrocytes isolated from human articular cartilage.The proportion of chondrocytes expressing p55 TNF-alpha receptor was significantly higher in populations from OA cartilage (p0.01) when compared with similar populations from non-arthritic cartilage. The number of p55 receptors/chondrocyte was also significantly higher (p0.01) in OA. A significant correlation (r = 0.72, p0.18) was found in OA between the numbers of chondrocytes expressing p75 receptor and the number of receptors/chondrocyte.p55 TNF-alpha receptor expression is significantly increased on OA chondrocytes ex vivo. Enhanced expression of p55, purported to be the biologically active receptor, could contribute to OA cartilage degradation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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