Kinetics of elementary steps in the cytochrome P-450 reaction sequence. II. Temperature dependence and species differences in substrate binding reaction

Autor:	G, Smettan, J, Blanck, G R, Jänig, K, Ruckpaul
Rok vydání:	1978
Předmět:	Kinetics Cytochrome P-450 Enzyme System Microsomes Liver Temperature Animals Rabbits Calorimetry Protein Binding Rats Substrate Specificity
Zdroj:	Acta biologica et medica Germanica. 37(4)
ISSN:	0001-5318
Popis:	Species dependencies and the temperature function of substrate binding reaction have been studied. The solubilized P-450 preparations from rat and rabbit, respectively, exhibit similar substrate binding characteristics with respect to rate constants and substrate specificity. The rabbit P-450 is more sensitive to preparational disintegration, this holds especially for aniline. In the Arrhenius plots a normal temperature dependence without breaks is observed. Iron ligands are bound with relatively low activation energies and negative entropies. The parameters increase for benzphetamine (type 1) and further for aniline (type 2), the latter substrate being entropically favoured too.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9623f19f858bc11db5ee298dd309934d https://pubmed.ncbi.nlm.nih.gov/735623 Zobrazit plný text záznamu