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To investigate the effect and mechanism of a novel emodin derivative YX-18 on Burkitt lymphoma (BL) cells.MTT assay was used to detect the effect of YX-18 on the proliferation of BL cell lines CA46 and Raji. Annexin V-PE/7-AAD double staining assay was used for detecting the effect of YX-18 on the apoptosis of CA46 and Raji cells. PI/RNase staining was used to test the effect of YX-18 on CA46 and Raji cell cycle. JC-1 method was used to measure the changes of mitochondrial membrane potential after YX-18 treatment, and DAPI staining was used to detect the morphology of apoptotic cells. Western blot was used to analyze the distribution changes of NF-κB pathway protein (P65, P-P65, IκB, P-IκB) in the cytoplasm and cell nucleus, and also the expression changes of cyclin-related protein P21, CDK2, P-CDK2, Cycling D1, Cycling E1, and the apoptosis-related protein Caspase-3, Caspase-8, Caspase-9 and the proliferation-related protein C-MYC, BCL-2 by YX-18. Real-time fluorescence-quantitative PCR was used to evaluate the effects of YX-18 on mRNA levels of C-MYC and Ki-67 genes in CA46 and Raji cells, and EBNA-1 and EBER genes of EBV in Raji (EBVNovel Emodin derivative YX-18 could effectively inhibit the proliferation of BL cell lines CA46 and Raji, showing a time-dependent effect (24, 48 and 72 h: rThe novel emodin derivative YX-18 can significantly inhibit the proliferation of Burkitt lymphoma cells, and induce the cell apoptosis and cycle arrest. The inhibitory effect of YX-18 on the proliferation of Burkitt lymphoma cells may be related with the effect of Caspase apoptosis pathway, the proliferation and apoptosis-related molecules, such as C-MYC and Ki-67, and also to the inhibition of NF-κB pathway.大黄素衍生物对伯基特淋巴瘤细胞株周期、凋亡、NF-κB通路的作用研究.探讨新型大黄素衍生物YX-18对伯基特淋巴瘤（BL）细胞的作用和机制.不同浓度YX-18 分别作用BL细胞株CA46和Raji细胞，MTT法检测YX-18对BL细胞株CA46和Raji增殖的影响，Annexin V-PE/7-AAD双染法检测YX-18对CA46和Raji细胞凋亡的影响，PI/RNase染色法检测YX-18对CA46和Raji细胞周期的影响，JC-1法检测YX-18作用后细胞线粒体膜电位的变化及DAPI染色检测细胞凋亡形态，Western blot检测YX-18对NF-κB通路蛋白（P65、P-P65、IκB、P-IκB）在胞浆和胞细胞核中的分布变化，细胞周期相关蛋白P21、CDK2、P-CDK2、Cycling D1、Cycling E1以及凋亡、增殖相关蛋白Caspase-3、Caspase-8、Caspase-9、C-MYC、BCL-2的表达变化，实时荧光定量PCR技术检测YX-18对CA46、Raji 细胞的C-MYC、Ki-67的 mRNA和Raji （EBV大黄素衍生物YX-18能有效抑制BL细胞株CA46和Raji的增殖并呈时间依赖效应（24, 48和72 h分别 r新型大黄素衍生物YX-18可以显著抑制BL细胞的增殖，同时诱导细胞凋亡并引起细胞周期阻滞。YX-18的作用可能与影响Caspase-分子和C-MYC、Ki-67等增殖凋亡相关分子，抑制NF-κB通路等有关. |
