| Popis: |
Using radioactive tracers, the effect of n-alkanes with a varying length--C16-C25--of the carbon chain on the rate of yeast growth and the ratio of major chemicals in their biomass was studied. The change in the level of n-alkanes accumulated by the yeast during carbon starvation was determined. No differences in the rate of metabolization of 1-14C-octadecane, 1,2-3H-hexadecane, and 13-14C-pentacosane were found. During 3 hour incubation the cell losses of C25, C18 and C16 amounted to 35.0, 33.0 and 38.1 microgram alkane/mg yeast, respectively. The intensity of yeast growth in the accumulative culture on dispersed n-alkanes with different molecular weights was similar. The specific formation of CO2 during the lag-phase by the yeast cultivated C18 was larger than on C23 or C25. The lipid content in the yeast grown on C18 was 3 times greater than in those grown on C22. The difference was made by an increased content of triglycerides and waxes. |
