Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [
| Autor: | J, Yankam Njiwa, N, Costes, C, Bouillot, S, Bouvard, S, Fieux, G, Becker, E, Levigoureux, G, Kocevar, C, Stamile, J B, Langlois, R, Bolbos, C, Bonnet, L, Bezin, L, Zimmer, A, Hammers |
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| Rok vydání: | 2016 |
| Předmět: |
Male
status epilepticus Epilepsy Pilocarpine Brain Original Articles Isoquinolines Receptors GABA-A Magnetic Resonance Imaging Epileptogenesis neuroinflammation Rats Sprague-Dawley Disease Models Animal Positron-Emission Tomography Animals Carbon Radioisotopes Longitudinal Studies Microglia Carrier Proteins TSPO Protein Binding |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism |
| ISSN: | 1559-7016 |
| Popis: | Inflammation may play a role in the development of epilepsy after brain insults. [11C]-(R)-PK11195 binds to TSPO, expressed by activated microglia. We quantified [11C]-(R)-PK11195 binding during epileptogenesis after pilocarpine-induced status epilepticus (SE), a model of temporal lobe epilepsy. Nine male rats were studied thrice (D0-1, D0 + 6, D0 + 35, D0 = SE induction). In the same session, 7T T2-weighted images and DTI for mean diffusivity (MD) and fractional anisotropy (FA) maps were acquired, followed by dynamic PET/CT. On D0 + 35, femoral arterial blood was sampled for rat-specific metabolite-corrected arterial plasma input functions (AIFs). In multiple MR-derived ROIs, we assessed four kinetic models (two with AIFs; two using a reference region), standard uptake values (SUVs), and a model with a mean AIF. All models showed large (up to two-fold) and significant TSPO binding increases in regions expected to be affected, and comparatively little change in the brainstem, at D0 + 6. Some individuals showed increases at D0 + 35. AIF models yielded more consistent increases at D0 + 6. FA values were decreased at D0 + 6 and had recovered by D0 + 35. MD was increased at D0 + 6 and more so at D0 + 35. [11C]-(R)-PK11195 PET binding and MR biomarker changes could be detected with only nine rats, highlighting the potential of longitudinal imaging studies. |
| Databáze: | OpenAIRE |
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