| Autor: |
Sunao, Sugita, Youichi, Iwanaga, Tatsushi, Kawaguchi, Yuri, Futagami, Shintaro, Horie, Tomoka, Usui, Sayaka, Yamamoto, Yoshiharu, Sugamoto, Manabu, Mochizuki, Norio, Shimizu, Ken, Watanabe, Miki, Mizukami, Tomohiro, Morio |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Nippon Ganka Gakkai zasshi. 112(1) |
| ISSN: |
0029-0203 |
| Popis: |
The human herpesvirus (HHV) family consists of types 1 to 8 (HHV1-8). The purpose of this study was to investigate the detection of HHV DNA, especially HSV1 (herpes simplex virus 1, HHV1), HSV2 (herpes simplex virus 2, HHV2), and VZV (varicella-zoster virus, HHV3) in ocular fluids of patients with acute retinal necrosis(ARN).The intraocular genome for HHV1-8 was determined in 19 ocular fluid samples (12 vitreous fluid and 7 aqueous humor samples) taken from ARN patients (n=14). The samples were tested for the presence of virus DNA by two systems of polymerase chain reaction (PCR): the multiplex PCR screening test and real-time quantitative PCR.Multiplex PCR demonstrated VZV (n=16, 84%), HSV1 (n = 1.5%) or HSV2 (n = 2.11%)genomic DNA in all the samples. In real-time PCR, a high copy number of virus DNA was detected. The virus DNA-positive samples contained Epstein-Barr virus (EBV, HHV4) DNA in 9 of 19 samples (47%). No HHV6-8 DNA was detected in the ocular samples, and no virus DNA was detected in the serum samples.The genome for HHV1-3 was detected in the patients with ARN. All cases contained a high copy number for the virus DNA that indicates viral replication. PCR systems are useful for determing whether virus infections are associated with uveitis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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