| Autor: |
Xiao-Dong, Cheng, Heng-Yue, Xie, Ruo-Lan, Qian |
| Rok vydání: |
2002 |
| Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 31(4) |
| ISSN: |
0582-9879 |
| Popis: |
A human erythroleukemia cell line(K562 cells)was used as a model to study the effect of interleukin-3(IL-3) on human globin gene expression in the cells. The results showed that the beta-globin gene was not expressed in uninduced K562 cells however, it was expressed when K562 cells were induced for 3 or 5 days by IL-3. The expression of alpha- and gamma-globin gene were not much different between IL-3 induced and uninduced K562 cells. Using the method of benzidine-dyeing, it was observed that the percentage of blue cells was significantly increased when K562 cells were induced by IL-3. It suggested that IL-3 could not only activate the exp-ression of beta-globin gene in K562 cells, but also induce the synthesis of hemoglobin. Therefore, IL-3 may play a critical role in inducing K562 cells to terminal differentiation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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