Interleukin-10 gene promoter polymorphism in Polish rheumatoid arthritis patients
| Autor: | A, Paradowska-Gorycka, J, Trefler, J, Maciejewska-Stelmach, J K, Łacki |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Genotype Middle Aged Polymerase Chain Reaction Polymorphism Single Nucleotide Severity of Illness Index Interleukin-10 Arthritis Rheumatoid Gene Frequency Risk Factors Surveys and Questionnaires Humans Female Genetic Predisposition to Disease Poland Promoter Regions Genetic Polymorphism Restriction Fragment Length Aged |
| Zdroj: | International journal of immunogenetics. 37(4) |
| ISSN: | 1744-313X |
| Popis: | Interleukin (IL)-10 is an important multifunctional cytokine with both anti-inflammatory and immunoregulatory effects in rheumatoid arthritis (RA). In the present study, we evaluated the frequency and potential impact of IL-10 promoter polymorphisms on susceptibility to and severity of RA in Polish in - patients with a high disease activity (mean DAS 28 C-reactive protein 5.25). DNA was obtained from 244 RA patients and 106 healthy controls. The -592C/A and -1082G/A IL-10 gene polymorphisms were amplified by polymerase chain reaction with restriction endonuclease mapping. The frequency of the IL-10-592CA, -592AA genotypes (respectively: 30% vs 5% and 7% vs 0%) and allele -592A (37% vs 5%) were significantly higher in RA patients as compared with a control group. We did not find any association of the IL-10-592C/A genotype distribution with disease parameters, except for an increased ESR (erythrocyte sedimentation rate) in patients with the -592CC genotype as compared with those with -592CA or -592AA genotypes (P = 0.01). The frequency of the IL-10-1082GG genotype was lower (P = 0.0001), and that of the IL-10-1082GA genotype was higher (P = 0.009) in RA patients comparing with the control group. In RA patients with -1082GA or -1082AA genotypes the time duration of the disease (P = 0.03), Health Assessment Questionnaire (HAQ) Score (P = 0.04) and PLT count (P = 0.001) were significantly increased as compared with subjects with -1082GG genotype. Presented findings indicate that IL-10-592C/A and IL-10-1082G/A polymorphisms may be considered genetic risk factors for RA susceptibility and severity. |
| Databáze: | OpenAIRE |
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