High level synthesis of immunoglobulins in Chinese hamster ovary cells
| Autor: | C R, Wood, A J, Dorner, G E, Morris, E M, Alderman, D, Wilson, R M, O'Hara, R J, Kaufman |
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| Rok vydání: | 1990 |
| Předmět: |
Base Sequence
Immunoglobulin mu-Chains Complement Fixation Tests Molecular Sequence Data Immunoglobulins DNA Transfection Antibodies Recombinant Proteins Cell Line Nitrophenols Cricetulus Immunoglobulin lambda-Chains Antibody Specificity Cricetinae Animals Cloning Molecular Carrier Proteins Genetic Engineering Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins Molecular Chaperones Phenylacetates |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 145(9) |
| ISSN: | 0022-1767 |
| Popis: | The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins. |
| Databáze: | OpenAIRE |
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