Vaccination with BV8S2 protein amplifies TCR-specific regulation and protection against experimental autoimmune encephalomyelitis in TCR BV8S2 transgenic mice
| Autor: | H, Offner, K, Adlard, B F, Bebo, J, Schuster, G G, Burrows, A C, Buenafe, A A, Vandenbark |
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| Rok vydání: | 1998 |
| Předmět: |
Immunosuppression Therapy
Male Encephalomyelitis Autoimmune Experimental Cell-Free System Receptors Antigen T-Cell alpha-beta Freund's Adjuvant Vaccination Epitopes T-Lymphocyte Mice Transgenic Myelin Basic Protein Th1 Cells Lymphocyte Activation Coculture Techniques Peptide Fragments Cell Line Rats Mice Inbred C57BL Mice Adjuvants Immunologic T-Lymphocyte Subsets Animals Female Lymph Nodes Transgenes |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 161(5) |
| ISSN: | 0022-1767 |
| Popis: | TCR determinants overexpressed by autopathogenic Th1 cells can naturally induce a second set of TCR-specific regulatory T cells. We addressed the question of whether immune regulation could be induced naturally in a genetically restricted model in which a major portion of TCR-specific regulatory T cells expressed the same target TCR BV8S2 chain as the pathogenic T cells specific for myelin basic protein (MBP). We found vigorous T cell responses to BV8S2 determinants in naive mice that could be further potentiated by vaccination with heterologous BV8S2 proteins, resulting in the selective inhibition of MBP-specific Th1 cells and protection against experimental encephalomyelitis. Moreover, coculture with BV8S2-specific T cells or their supernatants reduced proliferation, IFN-gamma secretion, and encephalitogenic activity of MBP-specific T cells. These results suggest that immune regulation occurs through a nondeletional cytokine-driven suppressive mechanism. |
| Databáze: | OpenAIRE |
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